Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 428977
Title Construction of recombinant Newcastle disease virus Italien strain for oncolytic virotherapy of tumors
Author(s) Wei, D.; Sun, N.; Nan, G.; Wang, Y.; Liu, H.Q.; Peeters, B.P.H.; Chen, Z.N.; Bian, H.
Source Human Gene Therapy 23 (2012)7. - ISSN 1043-0342 - p. 700 - 710.
DOI https://doi.org/10.1089/hum.2011.207
Department(s) CVI Virology
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) protein cleavage site - hepatitis-c virus - fusion protein - cancer-therapy - vaccine vector - foreign gene - phase-i - pv701 - expression - infection
Abstract Newcastle disease virus (NDV) is a naturally oncolytic virus that has been shown to be safe and effective for cancer therapy. Tumor virotherapy using NDV emerged in the 1950s and has advanced more recently by the increased availability of reverse genetics technology. In this study, we constructed a reverse genetics system based on the virulent and oncolytic NDV Italien strain, and generated two recombinant NDVs carrying a gene encoding either enhanced green fluorescent protein or firefly luciferase. We evaluated the replication and antitumor characteristics of these viruses in vitro and in vivo. Our data showed that the insertion of exogenous reporter genes did not affect NDV replication and sensitivity to type I interferon. The recombinant NDVs kept the property of tumor-selective replication both in vitro and in vivo and strongly induced syncytium formation leading to cell death. Moreover, the recombinant NDVs significantly prolonged the survival of tumor-bearing athymic mice (p=0.017) and suppressed the loss of body weight after intratumoral injection. Taken together, our study provides a novel platform to develop recombinant oncolytic viruses based on the NDV Italien strain and shows the efficiency of recombinant NDV Italien for oncolytic virotherapy of tumors.
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