Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 429026
Title Oit/Fam3D, a gut secreted protein displaying nutritional status-dependent regulation
Author(s) Wit, N.J.W. de; IJssenagger, N.; Oosterink, E.; Keshtkar, S.; Hooiveld, G.J.E.J.; Mensink, R.P.; Hammer, S.; Smit, J.W.A.; Muller, M.R.; Meer, R. van der
Source Journal of Nutritional Biochemistry 23 (2012)11. - ISSN 0955-2863 - p. 1425 - 1433.
DOI https://doi.org/10.1016/j.jnutbio.2011.09.003
Department(s) Chair Nutrition Metabolism and Genomics
Human Nutrition (HNE)
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) small-intestine - gene-expression - hormones
Abstract Oncoprotein-induced transcript 1 (Oit1) was previously identified as a dietary fat-induced gene in the small intestine of C57Bl/6J mice. In this study, we further characterized Oit1 and its human ortholog family with sequence similarity 3, member D (Fam3D), on the messenger RNA as well as the protein level. Oit1 and Fam3D were found to be predominantly expressed in the gastrointestinal tract of mice and humans, respectively. Dietary fat induced a clear and acute up-regulation of Oit1, especially in the jejunum, whereas fasting led to a reduced gene expression in the small intestine. Regarding protein expression, we found a remarkable pattern of Oit1 along the longitudinal axis of the intestine, a predominant villus-restricted expression in the proximal small intestine and a more pronounced crypt expression in the distal parts of the intestine. Using transfection experiments, we confirmed secretion of the Oit1 protein, as was predicted by a signal peptide sequence. Detection of Oit1 and Fam3D in plasma samples indicated that both proteins are secreted to the basolateral site of enterocytes. Moreover, in human plasma samples, we also found an effect of nutritional status on Fam3D levels, with a postprandial elevation and a reduction after fasting. In conclusion, Oit1 and Fam3D are gut-derived proteins that are expressed and secreted in a nutritional status-dependent manner.
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