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Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Record number 429321
Title Detection and characterization of pCT-like plasmid vectors for blaCTX-M-14 in Escherichia coli isolates from humans, turkeys and cattle in England and Wales
Author(s) Stokes, M.O.; Cottel, J.L.; Piddock, L.J.; Wu, G.; Wootton, M.; Mevius, D.J.; Randall, L.P.; Teale, C.J.; Fielder, M.D.; Coldham, N.G.
Source Journal of Antimicrobial Chemotherapy 67 (2012)7. - ISSN 0305-7453 - p. 1639 - 1644.
Department(s) CVI Bacteriology and Epidemiology
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) spectrum-beta-lactamase - ctx-m - salmonella - ctx-m-14 - enterobacteriaceae - resistance - strains - france - gene - uk
Abstract Objectives - To detect and characterize Escherichia coli strains and pCT-like plasmids implicated in the dissemination of the CTX-M-14 gene in animals and humans, in England and Wales. Methods UK CTX-M-14-producing E. coli (n¿=¿70) from cattle (n¿=¿33), turkeys (n¿=¿9), sheep (n¿=¿2) and humans (n¿=¿26) were screened using multiplex PCR for the detection of a previously characterized plasmid, pCT. Isolates found to be carrying two or more pCT genetic markers were further analysed using PFGE. Their antimicrobial-resistance genes and virulence genes were also determined. These plasmids were transferred to Salmonella enterica serotype Typhimurium 26R and further examined for incompatibility type, genetic environment of the blaCTX-M-14 gene, size, restriction fragment length polymorphism (RFLP) and nikB sequence. Results - The 25 E. coli isolates carrying pCT genetic markers generated 19 different PFGE profiles, and 23 isolates had different virulence and antimicrobial-resistance gene patterns. One isolate from cattle was a verotoxigenic E. coli (‘VTEC’); the rest were commensal or extra-intestinal pathogenic E. coli. pCT-like plasmids with similar molecular characteristics (size, replicon type, RFLP pattern, pCT markers and genetic environment of the blaCTX-M-14 gene) were detected in 21/25 of the field isolates, which comprised those from cattle (n¿=¿9), turkeys (n¿=¿8) and humans (n¿=¿4). All pCT-like plasmids were conjugative, and most were IncK (n¿=¿21) and had the same local genetic environment flanking the blaCTX-M-14 gene (n¿=¿23). RFLP analysis demonstrated =75% similarity among most plasmids (n¿=¿22). Conclusions - pCT-like plasmids were common vectors for horizontal dissemination of 30% of the blaCTX-M-14 genes to
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