Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 429417
Title European ring trial to evaluate ELISAs for the diagnosis of infection with Rift Valley fever virus
Author(s) Kortekaas, J.A.; Kant, J.; Vloet, R.P.M.; Cêtre-Sossah, C.; Marianneau, P.; Lacote, S.; Banyard, A.C.; Jeffries, C.; Eiden, M.; Groschup, M.; Jäckel, S.; Hevia, E.; Brun, A.
Source Journal of Virological Methods 187 (2013)1. - ISSN 0166-0934 - p. 177 - 181.
DOI http://dx.doi.org/10.1016/j.jviromet.2012.09.016
Department(s) CVI Virology
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) linked-immunosorbent-assay - reverse transcription-pcr - nucleocapsid protein - domestic ruminants - capture elisa - igg antibody - validation - sandwich - humans - sheep
Abstract A ring trial was organized to evaluate Rift Valley fever virus (RVFV) ELISAs by European laboratories. A total of five ELISAs, two of which specific for IgM antibodies, were evaluated by six participants. Sera were derived from cattle or sheep and originated from either a RVFV endemic area, a RVFV-free area or from experimental infection studies. Cohen's kappa analysis showed higher than 90% agreement of two commercially available ELISAs with the virus neutralization test, suggesting that primary screening as well as serological confirmation using these ELISAs is feasible. More extensive validations with sera of known IgM status are, however, required to determine agreement between IgM ELISAs.
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