Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Record number 429686
Title Pitfalls in the desulphation of glucosinolates in a high-throughput assay
Author(s) Hennig, K.; Verkerk, R.; Bonnema, A.B.; Dekker, M.
Source Food Chemistry 134 (2012)4. - ISSN 0308-8146 - p. 2355 - 2361.
DOI https://doi.org/10.1016/j.foodchem.2012.04.015
Department(s) Product Design and Quality Management Group
Laboratory of Plant Breeding
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) brassica-oleracea - broccoli - vegetables - breakdown - napus - hplc - food - ms
Abstract Glucosinolates are phytochemicals with health promoting properties. Determination as desulpho-glucosinolates is widely used and adesulphation in microtiter plates has been applied to reach highthroughput. The use of various sulphatase concentrations and volumes throughout literature necessitates the identification of an appropriate desulphation procedure in microtiter plates. High sulphatase concentrations (¿15 mg/ml) decreased the concentration of the internal standard glucotropaeolin, whereas the other glucosinolates were less affected. Due to the calculation based on the recovery of glucotropaeolin, this leads to an overestimation of GL concentrations after desulphation with high sulphatase concentrations. A glucosidase side-activity, present in the crude sulphatase powder, is likely causing this phenomenon. At lower sulphatase concentrations (1 mg/ml) glucoiberin and glucoraphanin were insufficiently desulphated. Combining these effects results in a small range of applicable sulphatase concentrations. A purified sulphatase preparation resulted in good recoveries for a diversity of samples and is hence recommended for highthroughputdesulphation in microtiter plates.
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