Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 429714
Title Self-Assembly of Silk-Collagen-Like Triblock Copolymers Resembles a Supramolecular Living Polymerization
Author(s) Beun, L.H.; Beaudoux, X.J.; Kleijn, J.M.; Wolf, F.A. de; Cohen Stuart, M.A.
Source ACS Nano 6 (2012)1. - ISSN 1936-0851 - p. 133 - 140.
Department(s) Physical Chemistry and Colloid Science
FBR Bioconversion
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) fibrils
Abstract We produced several pH-responsive silk-collagen-like triblocks, one acidic and two alkaline. At pH values where the silk-like block is uncharged the triblocks self-assemble into filaments. The pH-induced self-assembly was examined by atomic force microscopy, light scattering, and circular dichroism. The populations of filaments were found to be very monodisperse, indicating that the filaments start to grow from already present nuclei in the sample. The growth then follows pseudo-first-order kinetics for all examined triblocks. When normalized to the initial concentration, the growth curves of each type of triblock overlap, showing that the self-assembly is a generic process for silk-collagen-silk triblocks, regardless of the nature of their chargeable groups. The elongation speed of the filaments is slow, due to the presence of repulsive collagen-like blocks and the limited number of possibilities for an approaching triblock to successfully attach to a growing end. The formation of filaments is fully reversible. Already present filaments can start growing again by addition of new triblocks. The structure of all filaments is very rich in ß-turns, leading to ß-rolls. The triblocks attain this structure only when attaching to a growing filament.
Comments
There are no comments yet. You can post the first one!
Post a comment
 
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.