Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 429715
Title Tuning of Collagen Triple-Helix Stability in Recombinant Telechelic Polymers
Author(s) Silva, C.I. da; Skrzeszewska, P.J.; Golinska, M.D.; Werten, M.W.T.; Eggink, G.; Wolf, F.A. de
Source Biomacromolecules 13 (2012)5. - ISSN 1525-7797 - p. 1250 - 1258.
Department(s) Bioprocess Engineering
Physical Chemistry and Colloid Science
FBR Bioconversion
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) triblock copolymers - stabilization - hydrogels - gelatin - gels - hydroxylation - proteins - kinetics - network - water
Abstract The melting properties of various triblock copolymers with random coil middle blocks (100–800 amino acids) and triple helix-forming (Pro-Gly-Pro)n end blocks (n = 6–16) were compared. These gelatin-like molecules were produced as secreted proteins by recombinant yeast. The investigated series shows that the melting temperature (Tm) can be genetically engineered to specific values within a very wide range by varying the length of the end block. Elongation of the end blocks also increased the stability of the helices under mechanical stress. The length-dependent melting free energy and Tm of the (Pro-Gly-Pro)n helix appear to be comparable for these telechelic polymers and for free (Pro-Gly-Pro)n peptides. Accordingly, the Tm of the polymers appeared to be tunable independently of the nature of the investigated non-cross-linking middle blocks. The flexibility of design and the amounts in which these nonanimal biopolymers can be produced (g/L range) create many possibilities for eventual medical application.
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