Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 430344
Title NanoSIMS50 - a powerful tool to elucidate cellular localization of halogenated organic compounds
Author(s) Gutleb, A.C.; Freitas, J. de; Murk, A.J.; Verhaegen, S.; Ropstad, E.; Udelhoven, T.; Hoffmann, L.; Audinot, J.N.
Source Analytical and Bioanalytical Chemistry 404 (2012)9. - ISSN 1618-2642 - p. 2693 - 2698.
DOI https://doi.org/10.1007/s00216-012-6066-8
Department(s) Sub-department of Toxicology
WIMEK
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) in-vitro - perfluorinated compounds - h295r steroidogenesis - t-screen - assay - visualization - metabolites - transport - exposure - liver
Abstract Persistent organic pollutants are widely distributed in the environment and lots of toxicological data are available. However, little is known on the intracellular fate of such compounds. Here a method applying secondary ion mass spectrometry is described that can be used to visualize cellular localization of halogenated compounds and to semi-quantitatively calculate concentrations of such compounds. Of the model compounds tested, TBBPA was homogenously distributed in the cell membrane of the H295R cells while PFOS accumulated in very distinct locations in the cell membrane. Relative intracellular concentrations of 4-OH-BDE69 and 4-OH-BDE121 in GH3.TRE were 61 % and 18 %, respectively, compared to the parent compounds. These differences may partly explain that observed effect concentrations for 4-OH-BDEs in in vitro experiments are usually lower than what would be expected based on receptor binding studies. NanoSIMS50 proved to be a powerful tool to describe the cellular distribution of halogenated compounds. The semi-quantitative data that can be obtained may help to further explain results from in vitro or in vivo experiments
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