Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 430408
Title Complexes with mixed primary and secondary cellulose synthases are functional in Arabidopsis plants
Author(s) Carroll, A.; Mansoori Zangir, N.; Li, S.; Lei, L.; Vernhettes, S.; Visser, R.G.F.; Somerville, C.; Gu, Y.; Trindade, L.M.
Source Plant Physiology 160 (2012)2. - ISSN 0032-0889 - p. 726 - 737.
DOI http://dx.doi.org/10.1104/pp.112.199208
Department(s) Laboratory of Plant Breeding
WUR Plant Breeding
EPS
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) cell-wall synthesis - bimolecular fluorescence complementation - proteins in-vivo - cesa gene family - plasma-membrane - split-ubiquitin - populus-trichocarpa - biosynthesis - expression - visualization
Abstract In higher plants, cellulose is synthesized by so-called rosette protein complexes with cellulose synthases (CESAs) as catalytic subunits of the complex. The CESAs are divided into two distinct families, three of which are thought to be specialized for the primary cell wall and three for the secondary cell wall. In this article, the potential of primary and secondary CESAs forming a functional rosette complex has been investigated. The membrane-based yeast two-hybrid and biomolecular fluorescence systems were used to assess the interactions between three primary (CESA1, CESA3, CESA6), and three secondary (CESA4, CESA7, CESA8) Arabidopsis (Arabidopsis thaliana) CESAs. The results showed that all primary CESAs can physically interact both in vitro and in planta with all secondary CESAs. Although CESAs are broadly capable of interacting in pairwise combinations, they are not all able to form functional complexes in planta. Analysis of transgenic lines showed that CESA7 can partially rescue defects in the primary cell wall biosynthesis in a weak cesa3 mutant. Green fluorescent protein-CESA protein fusions revealed that when CESA3 was replaced by CESA7 in the primary rosette, the velocity of the mixed complexes was slightly faster than the native primary complexes. CESA1 in turn can partly rescue defects in secondary cell wall biosynthesis in a cesa8ko mutant, resulting in an increase of cellulose content relative to cesa8ko. These results demonstrate that sufficient parallels exist between the primary and secondary complexes for cross-functionality and open the possibility that mixed complexes of primary and secondary CESAs may occur at particular times.
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