Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 430540
Title Preservation of seed viability during 25 years of storage under standard genebank conditions
Author(s) Treuren, R. van; Groot, E.C. de; Hintum, T.J.L. van
Source Genetic Resources and Crop Evolution 60 (2013)4. - ISSN 0925-9864 - p. 1407 - 1421.
DOI https://doi.org/10.1007/s10722-012-9929-0
Department(s) Centrum voor Genetische Bronnen Nederland
Centre for Genetic Resources, The Netherlands
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) desiccation-tolerance - longevity - brassicaceae - germination - maturity
Abstract Maintaining sufficient viability is critical to the sustainability of ex situ conserved seed collections. For this reason, accessions are regenerated when viability falls below a predefined threshold. Viability is monitored by determining the germination ability of accessions at predefined time intervals. Optimizing the frequency of these germination tests, in order to avoid waste of resources, is hampered by the scarce availability of data about seed longevity, particularly for material maintained under genebank conditions. Here we report on the analysis of nearly 40,000 germination test results collected for a wide range of crop species over a 25-years period by the centre for genetic resources, the Netherlands (CGN), where seeds of genebank accessions are dried to 3–7 % moisture content and stored for the long term under near-vacuum in aluminium foil bags at -20 °C. The results indicate that seed viability is well maintained for the large majority of seed lots during the first 25 years after regeneration as only 3.3 % of the monitoring tests revealed below-threshold germination values. It is argued that the majority of these sub-standard seed lots are due to other causes than seed ageing, including dormancy problems and estimation error in germination testing. For material, maintained under the seed management procedures and storage conditions practiced by CGN, it is therefore recommended to delay the first germination monitoring tests to 25 years after regeneration.
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