Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 430571
Title Structure-informed analysis of Gpa2 and Rx1 recognition specificity
Author(s) Bakker, E.H.; Slootweg, E.J.; Roosien, J.; Butterbach, P.B.E.; Spiridon, L.N.; Petrescu, A.J.; Bakker, J.; Goverse, A.
Source In: The 31th International Symposium of the European Society of Nematologists, Adana, Turkey, 23-27 September 2012. - Adana, Turkey : - p. 166 - 166.
Event Adana, Turkey : The 31th International Symposium of the European Society of Nematologists, Adana, 2012-09-23/2012-09-27
Department(s) Laboratory of Nematology
Laboratory of Virology
EPS-2
Publication type Abstract in scientific journal or proceedings
Publication year 2012
Abstract The potato cyst nematode resistance gene Gpa2 confers resistance to Globodera pallida. Gpa2 belongs to the class of CC-NB-LRR resistance genes and is located on a complex locus that also harbours the closely related Potato Virus X (PVX) resistance gene Rx1. Rx1 and Gpa2 are 88% identical at the amino acid level, yet they confer resistance to completely unrelated pathogens. For both R proteins, the pathogen elicitor that triggers the resistance response is known. Gpa2 recognizes the secreted Globodera pallida protein RBP1, whereas Rx1 detects the PVX coat protein. This makes Gpa2 and Rx1 an excellent model system to investigate how the recognition specificity is determined in NB-LRR proteins. To determine which amino acids may play a role in Gpa2 and Rx1 specificity, we combined sequence information of 35 closely related homologues derived from wild Solanum species, functional data and a consensus in sillico 3D model obtained in previous studies. For both Gpa2 and Rx1, two residues were detected which are located in the region that is crucial for elicitor recognition. In the 3D model, they map together on the LRR surface, suggesting a potential role in pathogen recognition. The functional validation of these residues is in progress.
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