Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 430946
Title The relation between microbiota in water, feed and fish in different types of recirculation systems for turbot
Author(s) Bacanu, G.M.; Rurangwa, E.; Sipkema, D.; Smidt, H.
Event AQUA 2012 European Aquaculture Society and World Aquaculture Society Joint Meeting, Prague, Czech Republic, 2012-09-01/2012-09-05
Department(s) IMARES Aquaculture
Microbiological Laboratory
WIAS
Publication type Abstract in scientific journal or proceedings
Publication year 2012
Abstract Fish are continuously exposed to a wide range of microorganisms present in the environment. This might influence the intestinal microbiota which in turn influence animal health and contribute to nutrition. Two turbot farms each with a different recirculating system concept were monitored. The objective of this study was to identify and compare in time the microbiota in (i) inflow water, (ii) water in the rearing tanks, (iii) feed and (iv) fish guts. In fish guts, a distinction was made between microbiota collected from faeces (lumen) and microbiota attached to the gut wall (mucus). Juvenile turbot, water and feed were sampled every 3 months over a 1-year period. Combined analyses using 16SrRNA gene PCR amplification and denaturing gradient gel electrophoresis (DGGE) and 16SrRNA gene pyrosequencing data showed that the microbial community composition in water changed over time and was farm-dependent. Microbial DGGE fingerprints clustered by farm suggesting a strong farm effect (Figure 1). Pyrosequencing data confirmed differences in microbiota in faeces, gut mucus, water and feed (Figure 2). The microbial community in turbot gut showed high inter-individual variation and was much less affected by system design or management. The turbot gut microbiota clustered based on the sampling location (lumen or mucus). The feed had the lowest bacterial diversity. Moreover, microbiota composition in water, feed, and gut were more or less stable over time during the observation period, suggesting that the gut was almost fully developed by the time the turbot reached 15g at the start of the experiment.
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