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Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 431717
Title Characterisation and quantification of liposome-type nanoparticles in a beverage matrix using hydrodynamic chromatography and MALDI–TOF mass spectrometry
Author(s) Helsper, J.P.F.G.; Peters, R.J.B.; Brouwer, L.; Weigel, S.
Source Analytical and Bioanalytical Chemistry 405 (2013)4. - ISSN 1618-2642 - p. 1181 - 1189.
Department(s) PRI BIOS Applied Genomics & Proteomics
RIKILT - Business unit Contaminants & Toxins
RIKILT - Business unit Dierbehandelingsmiddelen
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) assisted laser-desorption - food
Abstract This paper describes the characterisation of liposome-type nanoparticles (NPs) dispersed in a beverage matrix. Characterisation is based on a two-step procedure: first, liposomes are separated on the basis of size in the nanometre range by use of hydrodynamic chromatography (HDC); second, chemical characterisation is performed by use of MALDI–TOF mass spectrometry (MS). Characterisation of three types of Coatsome liposome, a commercially available type of empty liposome, was investigated. All three liposome types, Coatsome A¿=¿anionic, N¿=¿neutral, and C¿=¿cationic, gave single peaks in HDC, reflecting diameters of 153, 187, and 205 nm, respectively. Subsequent MALDI–TOF MS in positive mode furnished major signals at m/z¿=¿734.5 ([M¿+¿H]+ adduct) and m/z¿=¿756.6 ([M¿+¿Na]+ adduct) of l-(a)-dipalmitoylphosphatidylcholine (DPPC) monomer and dimeric adducts at m/z¿=¿1468.1 and m/z¿=¿1490.1, respectively. MALDI–TOF MS in negative mode gave a signal at m/z¿=¿721.3 ([M¿-¿H]- adduct) of l-(a)-dipalmitoylphosphatidylglycerol (DPPG), except for Coatsome C which lacks this phospholipid. After HDC separation of Coatsome A NPs the major DPPC and DPPG signals can be detected in the expected fractions by use of MALDI–TOF MS in positive and negative modes, respectively. Validation of the analytical strategy revealed linearity (R2¿>¿0.99), repeatability (relative standard deviation
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