Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 431719
Title Structural annotation and elucidation of conjugated phenolic compounds in black, green, and white tea extracts
Author(s) Hooft, J.J.J. van der; Akermi, M.; Yelda Ünlü, F.; Mihaleva, V.; Gomez Roldan, V.; Bino, R.J.; Vos, R.C.H. de; Vervoort, J.J.M.
Source Journal of Agricultural and Food Chemistry 60 (2012)36. - ISSN 0021-8561 - p. 8841 - 8850.
DOI https://doi.org/10.1021/jf300297y
Department(s) PRI BIOS Applied Metabolic Systems
AFSG Staff Departments (FBR)
Laboratory of Plant Physiology
Biochemistry
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) pu-erh tea - mass-spectrometry - oolong tea - hplc-dad - lc-msn - liquid-chromatography - chlorogenic acids - camellia-sinensis - purine alkaloids - ellagic acid
Abstract Advanced analytical approaches consisting of both LC-LTQ-Orbitrap Fourier transformed (FT)-MS and LC-time-of-flight-(TOF)-MS coupled to solid-phase extraction (SPE) NMR were used to obtain more insight into the complex phenolic composition of tea. On the basis of the combined structural information from (i) accurate mass fragmentation spectra, derived by using LC-Orbitrap FTMS(n), and (ii) proton NMR spectra, derived after LC-TOFMS triggered SPE trapping of selected compounds, 177 phenolic compounds were annotated. Most of these phenolics were glycosylated and acetylated derivatives of flavan-3-ols and flavonols. Principal component analysis based on the relative abundance of the annotated phenolic compounds in 17 commercially available black, green, and white tea products separated the black teas from the green and white teas, with epicatechin-3,5-di-O-gallate and prodelphinidin-O-gallate being among the main discriminators. The results indicate that the combined use of LC-LTQ-Orbitrap FTMS and LC-TOFMS-SPE-NMR leads to a more comprehensive metabolite description and comparison of tea and other plant samples
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