Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 431961
Title Reverse breeding in Arabidopsis thaliana generates homozygous parental lines from a heterozygous plant
Author(s) Wijnker, T.G.; Dun, K.P.M. van; Snoo, C.B.; Lelivelt, C.L.C.; Keurentjes, J.J.B.; Naharudin, N.S.; Ravi, M.; Chan, S.W.L.; Jong, J.H.S.G.M. de; Dirks, R.
Source Nature Genetics 44 (2012). - ISSN 1061-4036 - p. 467 - 470.
DOI http://dx.doi.org/10.1038/ng.2203
Department(s) Laboratory of Genetics
EPS
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) genome - dna - construction - meiosis - genes - rflp - map
Abstract Traditionally, hybrid seeds are produced by crossing selected inbred lines. Here we provide a proof of concept for reverse breeding, a new approach that simplifies meiosis such that homozygous parental lines can be generated from a vigorous hybrid individual. We silenced DMC1, which encodes the meiotic recombination protein DISRUPTED MEIOTIC cDNA1, in hybrids of A. thaliana, so that non-recombined parental chromosomes segregate during meiosis. We then converted the resulting gametes into adult haploid plants, and subsequently into homozygous diploids, so that each contained half the genome of the original hybrid. From 36 homozygous lines, we selected 3 (out of 6) complementing parental pairs that allowed us to recreate the original hybrid by intercrossing. In addition, this approach resulted in a complete set of chromosome-substitution lines. Our method allows the selection of a single choice offspring from a segregating population and preservation of its heterozygous genotype by generating homozygous founder lines
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