Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 432032
Title Butyrate-producing Clostridium cluster XIVa species specifically colonize mucins in an in vitro gut model
Author(s) Abbeele, P. van den; Belzer, C.; Goossens, M.; Kleerebezem, M.; Vos, W.M. de; Thas, O.; Weirdt, R. de; Kerckhoffs, D.M.F.J.; Wiele, T. van der
Source ISME Journal 7 (2013). - ISSN 1751-7362 - p. 949 - 961.
DOI http://dx.doi.org/10.1038/ismej.2012.158
Department(s) Microbiological Laboratory
Host Microbe Interactomics
VLAG
WIMEK
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) inflammatory-bowel-disease - phylogenetic microarray analysis - gel-electrophoresis analysis - mucosa-associated microbiota - human large-intestine - 16s ribosomal-rna - fecal microbiota - sp-nov. - gastrointestinal-tract - spatial-organization
Abstract The human gut is colonized by a complex microbiota with multiple benefits. Although the surface-attached, mucosal microbiota has a unique composition and potential to influence human health, it remains difficult to study in vivo. Therefore, we performed an in-depth microbial characterization (human intestinal tract chip (HITChip)) of a recently developed dynamic in vitro gut model, which simulates both luminal and mucosal gut microbes (mucosal-simulator of human intestinal microbial ecosystem (M-SHIME)). Inter-individual differences among human subjects were confirmed and microbial patterns unique for each individual were preserved in vitro. Furthermore, in correspondence with in vivo studies, Bacteroidetes and Proteobacteria were enriched in the luminal content while Firmicutes rather colonized the mucin layer, with Clostridium cluster XIVa accounting for almost 60% of the mucin-adhered microbiota. Of the many acetate and/or lactate-converting butyrate producers within this cluster, Roseburia intestinalis and Eubacterium rectale most specifically colonized mucins. These 16S rRNA gene-based results were confirmed at a functional level as butyryl-CoA:acetate-CoA transferase gene sequences belonged to different species in the luminal as opposed to the mucin-adhered microbiota, with Roseburia species governing the mucosal butyrate production. Correspondingly, the simulated mucosal environment induced a shift from acetate towards butyrate. As not only inter-individual differences were preserved but also because compared with conventional models, washout of relevant mucin-adhered microbes was avoided, simulating the mucosal gut microbiota represents a breakthrough in modeling and mechanistically studying the human intestinal microbiome in health and disease. Finally, as mucosal butyrate producers produce butyrate close to the epithelium, they may enhance butyrate bioavailability, which could be useful in treating diseases, such as inflammatory bowel disease
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