Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 432266
Title Effect of galactooligosaccharides and Bifidobacterium animalis Bb-12 on growth of Lactobacillus amylovorus DSM 16698, microbial community structure, and metabolite production in an in vitro colonic model set up with human or pig microbiota
Author(s) Martinez, R.C.R.; Cardarelli, H.; Borst, W.; Albrecht, S.A.; Schols, H.A.; Gutierrez, O.; Maathuis, A.; Melo Franco, B.D. de; Martinis, E.C.P. de; Zoetendal, E.G.; Venema, K.; Saad, S.M.I.; Smidt, H.
Source FEMS Microbiology Ecology 84 (2013)1. - ISSN 0168-6496 - p. 110 - 123.
DOI https://doi.org/10.1111/1574-6941.12041
Department(s) Microbiological Laboratory
Food Chemistry Group
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) gradient gel-electrophoresis - 16s ribosomal-rna - large-intestine - fecal samples - human feces - gastrointestinal-tract - quantitative pcr - dietary-fibers - gut bacteria - double-blind
Abstract A validated in vitro model of the large intestine (TIM-2), set up with human or pig faeces, was used to evaluate the impact of potentially probiotic Lactobacillus amylovorus DSM 16698, administered alone (i), in the presence of prebiotic galactooligosaccharides (GOS) (ii), and co-administered with probiotic Bifidobacterium animalis ssp. lactis Bb-12 (Bb-12) (iii) on GOS degradation, microbial growth (L. amylovorus, lactobacilli, bifidobacteria and total bacteria) and metabolite production. High performance anion exchange chromatography revealed that GOS degradation was more pronounced in TIM-2 inoculated with pig faeces than with human faeces. Denaturing gradient gel electrophoresis profiling of PCR-amplified 16S rRNA genes detected a more complex Lactobacillus spp. community in pig faecal material than in human faecal inoculum. According to 16S rRNA gene-targeted qPCR, GOS stimulated the growth of lactobacilli and bifidobacteria in faecal material from both materials. The cumulative production of short chain fatty acids and ammonia was higher (P <0.05) for pig than for human faeces. However, lactate accumulation was higher (P <0.05) in the human model and increased after co-administration with GOS and Bb-12. This study reinforced the notion that differences in microbiota composition between target host organisms need to be considered when animal data are extrapolated to human, as is often done with pre- and probiotic intervention studies
Comments
There are no comments yet. You can post the first one!
Post a comment
 
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.