Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 432287
Title Review of Factors Affecting Organogenesis, Somatic Embryogenesis and Agrobacterium tumefaciens-Mediated Transformation of Strawberry
Author(s) Husaini, A.M.; Mercado, J.A.; Abdin, M.Z.; Teixeira da Silva, J.A.; Schaart, J.
Source In: Genes, Genomes and Genomics / Teixeira da Silva, J.A., Japan : Global Science Books (Genes, Genomes and Genomics 5) - p. 1 - 11.
Department(s) Laboratory of Plant Breeding
Publication type Chapter in scientific book
Publication year 2011
Abstract Invited Review: Standardization of an efficient regeneration system for each strawberry genotype is generally an indispensible pre-requisite for the successful development of transgenic plants. In this paper, we review some key factors affecting the regeneration of strawberry plants via adventitious organogenesis or somatic embryogenesis, such as type of explant, growth regulators or dark/light treatments. Since Agrobacterium tumefaciens-mediated transformation is the method of choice for strawberry transformation, we review the strategies adopted by different scientists to achieve higher transformation efficiencies and recovery of marker-free transgenic plants. Sufficient Agrobacterium cells during cocultivation, an adequate cocultivation period, the use of vir gene inducers like acetosyringone, introduction of a pre-selection phase between co-cultivation and selection, and optimum selection pressure, are all important factors to obtain stable transformants. For effective transformation, the antibiotic regime should control bacterial growth without inhibiting the regeneration of plant cells. A general protocol for the Agrobacterium transformation of strawberry leaf discs is also described. Finally, we discuss the metrics employed by different researchers for measuring the success of transformation, and highlight the difference between transformation efficiency and transformation percentage.
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