Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 432659
Title Comparison of three intestinal permeability methods
Author(s) Meer, Y. van der; Gerrits, W.J.J.; Bosch, M. van den; Moreto, M.; Buurman, W.A.; Kempen, T.A.T.G. van
Source In: Digestive Physiology of Pigs, book of abstracts, Keystone, CO, USA, May 29 - June 1, 2012. - Keystone, Co, USA : - p. 128 - 128.
Event Keystone, Co, USA : XII International symposium on digestive physiology of pigs, Keystone, CO, USA, May 29 - June 1, 2012, 2012-05-29/2012-06-01
Department(s) Animal Nutrition
Publication type Abstract in scientific journal or proceedings
Publication year 2012
Abstract The determination of intestinal permeability remains a challenge. The Ussing chamber is an ex vivo technique which is generally well accepted. However, this technique is tedious and requires a biopsy which can only be obtained through invasive surgery or post-mortem. It also represents a spot sample, sometimes less than 1 cm2. The everted gut sac technique uses a more relevant sample size, and in vivo permeability measurements using Co-EDTA or lactulose are much more practical. Our objective was to compare these 3 techniques. For this, 72 piglets were sampled either 3.5, 7.5, or 10.5 d post weaning. Both lactulose (1.3 g, 9.5 Å) and Co-EDTA (0.6 g, 10.0 Å) dissolved in 15 g demineralized water were administered intragastrically. Two hours later a blood sample was obtained through venipuncture. Subsequently, a 15 cm segment harvested at 25, 50, and 75% of the length of the small intestine was inverted, filled with phosphate buffered saline (PBS) containing 5 mM glucose, and sealed. Sacs were inserted for 60 min. in an aluminum foil covered bath with aerated PBS containing 125 µM FITC-dextran (14.0 Å) and maintained at 39°C after which both the sac content and medium were analyzed for FITC. Statistical analysis was performed using the Pearson correlation test of SPSS 19 (2010). The FITC analysis of the buffer revealed that the FITC concentrations dropped quickly (half-life ± 3 h), even though it was kept from contact with light by aluminum foil. This is raising doubts about the validity of the test. Serum concentrations of Co-EDTA and lactulose were positively correlated (r = 0.67, P <0.01). FITC dextran, corrected for a predicted change in half-life of stock solution, in contrast, did not correlate with either lactulose or Co-EDTA, for any of the segments compared. In conclusion, a correlation was found between Co-EDTA and lactulose suggesting that both marker molecules measure the same change in permeability, while ex vivo determinations using FITCdextran in everted gut sacs requires additional work before it can be applied.
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