Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 433122
Title Comparison between stearoyl-CoA desaturase expression in milk somatic cells and in mammary tissue of lactating dairy cows
Author(s) Jacobs, A.A.A.; Dijkstra, J.; Hendriks, W.H.; Baal, J. van; Vuuren, A.M. van
Source Journal of Animal Physiology and Animal Nutrition 97 (2013)2. - ISSN 0931-2439 - p. 353 - 362.
DOI http://dx.doi.org/10.1111/j.1439-0396.2012.01278.x
Department(s) Animal Nutrition
LR - Animal Nutrition
WIAS
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) conjugated linoleic-acid - epithelial-cells - messenger-rna - short communication - lipid-metabolism - gene-expression - linseed oil - gland - fat - cla
Abstract Stearoyl-CoA desaturase (SCD) is an important enzyme in the bovine mammary gland, where it inserts a cis-double bond at the ¿9 position in a wide range of fatty acids. Investigating SCD expression in the bovine mammary gland generally requires invasive biopsy to obtain mammary tissue. The aim of this study was to evaluate the use of milk somatic cells as a non-invasive alternative to biopsy for measuring mammary SCD expression in dairy cows. Both milk somatic cells and mammary tissue were collected from 14 Holstein-Friesian cows and used for analysis of SCD expression by real-time PCR. The SCD5 mRNA levels in mammary tissue compared with SCD1 were low, and for several milk somatic cell samples, SCD5 expression was even below the limit of detection. A significant relationship was found between SCD1 expression in milk somatic cells and in mammary tissue. In addition, SCD1 expression in milk somatic cells was significantly related to ¿9-desaturase indices in milk, which are commonly used as an indicator of SCD1 activity within the mammary gland. Our study showed that milk somatic cells can be used as a source of mRNA to study SCD1 expression in dairy cows, offering a non-invasive alternative to mammary tissue samples obtained by biopsy.
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