Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 433438
Title A novel method for viability enumeration for single-droplet drying of Lactobacillus plantarum WCFS1
Author(s) Perdana, J.A.; Bereschenko, L.A.; Roghair, M.; Fox, M.B.; Boom, R.M.; Kleerebezem, M.; Schutyser, M.A.I.
Source Applied and Environmental Microbiology 78 (2012)22. - ISSN 0099-2240 - p. 8082 - 8088.
DOI http://dx.doi.org/10.1128/AEM.02063-12
Department(s) Food Process Engineering
Microbiological Laboratory
Host Microbe Interactomics
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) acid starter cultures - industrial applications - comparative survival - phase-transitions - probiotics - microorganisms - mechanisms - diversity - bacteria - microencapsulation
Abstract Survival of probiotic bacteria during drying is not trivial. Survival percentages are very specific for each probiotic strain and can be improved by careful selection of drying conditions and proper drying carrier formulation. An experimental approach is presented, comprising a single-droplet drying method and a subsequent novel screening methodology, to assess the microbial viability within single particles. The drying method involves the drying of a single droplet deposited on a flat, hydrophobic surface under well-defined drying conditions and carrier formulations. Semidried or dried particles were subjected to rehydration, fluorescence staining, and live/dead enumeration using fluorescence microscopy. The novel screening methodology provided accurate survival percentages in line with conventional plating enumeration and was evaluated in single-droplet drying experiments with Lactobacillus plantarum WCFS1 as a model probiotic strain. Parameters such as bulk air temperatures and the carrier matrices (glucose, trehalose, and maltodextrin DE 6) were varied. Following the experimental approach, the influence on the viability as a function of the drying history could be monitored. Finally, the applicability of the novel viability assessment was demonstrated for samples obtained from drying experiments at a larger scale.
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