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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Record number 433550
Title Micropropagation of Ferula assa-foetida L. (a medicinal plant) via direct somatic embryogenesis
Author(s) Roozbeh, S.; Otroshy, M.; Bozorgipoor, R.; Ebrahimi, M.; Moeini Najafabadi, A.; Struik, P.C.
Source In: Proceedings IS on Medicinal and Aromatic Plants – IMAPS 2010 and “History of Mayan Ethnopharmacology” – IMAPS 2011. - - p. 143 - 152.
Event IS on Medicinal and Aromatic Plants – IMAPS 2010 and “History of Mayan Ethnopharmacology” – IMAPS 2011, 2011-11-20/2011-11-23
Department(s) Crop and Weed Ecology
Centre for Crop Systems Analysis
Publication type Contribution in proceedings
Publication year 2012
Abstract Ferula assa-foetida is a medicinal plant from the Apiaceae family. It is a monocarpic plant and only flowers once during its growth period and then it dries forever and that is one of the main problems of multiplication. The experiment was carried out to examine the effect of explants, medium and plant growth regulators, on the in vitro direct somatic embryogenesis of asafetida (Ferula assa-foetida). Explants of root, hypocotyl and cotyledon (leaf) were investigated on MS and B5 medium combined with 2,4-D (0.2, 0.5 or 1 mg/L), BAP (0.2, 0.5 or 1 mg/L) and NAA (0.2, 0.5 or 1 mg/L), each of them was combined with kin (0, 0.2, 0.5 or 1 mg/L). The best direct somatic embryogenesis was obtained from explants of leaves, no significant effect between explants of cotyledon and hypocotyls was found. 2,4-D (0.2 mg/L) + kin (0.2 mg/L) resulted in the highest percent of direct somatic embryogenesis, also the maximum indirect somatic embryogenesis was obtained from explants of root grown on the 2,4-D (0.5 mg/L) + kin (0.2 mg/L). The highest value for direct somatic embryogenesis was found in MS medium combined with 2,4-D (0.2 mg/L) + kin (0.2 mg/L), while the maximum of indirect somatic embryogenesis was observed in B5 medium enriched with 2,4-D (0.5 mg/L) + kin (0.2 mg/L). The maximum percentage of embryo conversion into seedling was found with 2,4-D (0.5 mg/L) + kin (0.2 mg/L) in B5. Highest percent of callogenesis was observed on B5 containing NAA (1 mg/L) + kin (1 mg/L), BAP (0.2 mg/L) + kin (0.5 mg/L) and BAP (0.5 mg/L) + kin (0.5 mg/L).
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