Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 434245
Title Evidence for functional diversification within a fungal NEP1-like protein family
Author(s) Santhanam, P.; Esse, H.P. van; Albert, I.; Faino, L.; Nürnberger, T.; Thomma, B.P.H.J.
Source Molecular Plant-Microbe Interactions 26 (2013)3. - ISSN 0894-0282 - p. 278 - 286.
DOI https://doi.org/10.1094/MPMI-09-12-0222-R
Department(s) Laboratory of Phytopathology
EPS-2
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) carotovora subsp carotovora - plant-pathogen interactions - necrosis-inducing activity - chitin-triggered immunity - verticillium-dahliae - mycosphaerella-graminicola - molecular characterization - arabidopsis-thaliana - fusarium-oxysporum - npp1 domain
Abstract In this study, we functionally analyzed the gene family encoding necrosis- and ethylene-inducing-like proteins (NLPs) of the vascular wilt pathogen Verticillium dahliae. We show that the composition of the NLP gene family varies little among V. dahliae isolates. The cytotoxic activity of NLP family members of a tomato pathogenic V. dahliae strain was determined, demonstrating that only two of the seven NLPs induced plant cell death. The genes encoding these cytotoxic NLPs were found to be induced in V. dahliae upon colonization of tomato. Interestingly, targeted deletion of either of the two genes in V. dahliae significantly compromised virulence on tomato as well as on Arabidopsis plants, whereas deletion of only one of the two genes affected virulence on N. benthamiana. This could be attributed to differential induction of the two NLP genes in V. dahliae upon N. benthamiana colonization, revealing that the in planta induction of NLP genes varies between plant hosts. Intriguingly, one of the NLP genes appears to also affect vegetative growth and conidiospore production, as the corresponding deletion strain produced significantly less conidiospores and developed extensive aerial mycelium. In conclusion, we demonstrate that the expanded V. dahliae NLP family shows functional diversification, not only revealing differential cytotoxicity between family members, but also that the cytotoxic NLPs play a role in vegetative growth and asexual reproduction in addition to their contribution to virulence.
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