Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 434464
Title Three-step pathway engineering results in more incidence rate and higher emission of nerolidol and improved attraction of Diadegma semiclausum
Author(s) Houshyani Hassanzadeh, B.; Assareh, M.; Busquets, A.; Ferrer, A.; Bouwmeester, H.J.; Kappers, I.F.
Source Metabolic Engineering 15 (2013). - ISSN 1096-7176 - p. 88 - 97.
DOI http://dx.doi.org/10.1016/j.ymben.2012.10.002
Department(s) Laboratory of Plant Physiology
Laboratory of Entomology
PE&RC
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) farnesyl-diphosphate synthase - arabidopsis-thaliana - terpenoid metabolism - nicotiana-attenuata - mass-spectrometry - lesion formation - plant volatiles - defense - biosynthesis - herbivores
Abstract The concentration and ratio of terpenoids in the headspace volatile blend of plants have a fundamental role in the communication of plants and insects. The sesquiterpene (E)-nerolidol is one of the important volatiles with effect on beneficial carnivores for biologic pest management in the field. To optimize de novo biosynthesis and reliable and uniform emission of (E)-nerolidol, we engineered different steps of the (E)-nerolidol biosynthesis pathway in Arabidopsis thaliana. Introduction of a mitochondrial nerolidol synthase gene mediates de novo emission of (E)-nerolidol and linalool. Co-expression of the mitochondrial FPS1 and cytosolic HMGR1 increased the number of emitting transgenic plants (incidence rate) and the emission rate of both volatiles. No association between the emission rate of transgenic volatiles and their growth inhibitory effect could be established. (E)-Nerolidol was to a large extent metabolized to non-volatile conjugates.
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