Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 434759
Title A diet high in resistant starch modulates microbiota composition, SCFA concentrations, and gene expression in pig intestine
Author(s) Haenen, D.; Zhang, J.; Souza Da Silva, C.; Bosch, G.; Meer, I.M. van der; Arkel, J. van; Borne, J.J.G.C. van den; Pérez Gutiérrez, O.; Smidt, H.; Kemp, B.; Müller, M.R.; Hooiveld, G.J.E.J.
Source The Journal of Nutrition 143 (2013)3. - ISSN 0022-3166 - p. 274 - 283.
DOI http://dx.doi.org/10.3945/jn.112.169672
Department(s) Chair Nutrition Metabolism and Genomics
Microbiological Laboratory
Adaptation Physiology
Animal Nutrition
PRI BIOS Applied Genomics & Proteomics
VLAG
WIAS
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) chain fatty-acids - glucagon-like peptide-1 - phylogenetic microarray - gastrointestinal-tract - human gut - appetite regulation - metabolic syndrome - colonic function - us adults - body-fat
Abstract Resistant starch (RS) is highly fermentable by microbiota in the colon, resulting in the production of SCFAs. RS is thought to mediate a large proportion of its health benefits, including increased satiety, through the actions of SCFAs. The aim of this study was to investigate the effects of a diet high in RS on luminal microbiota composition, luminal SCFA concentrations, and the expression of host genes involved in SCFA uptake, SCFA signaling, and satiety regulation in mucosal tissue obtained from small intestine, cecum, and colon. Twenty adult female pigs were either assigned to a digestible starch (DS) diet or a diet high in RS (34%) for a period of 2 wk. After the intervention, luminal content and mucosal scrapings were obtained for detailed molecular analysis. RS was completely degraded in the cecum. In both the cecum and colon, differences in microbiota composition were observed between DS- and RS-fed pigs. In the colon these included the stimulation of the healthy gut-associated butyrate-producing Faecalibacterium prausnitzii, whereas potentially pathogenic members of the Gammaproteobacteria, including Escherichia coli and Pseudomonas spp., were reduced in relative abundance. Cecal and colonic SCFA concentrations were significantly greater in RS-fed pigs, and cecal gene expression of monocarboxylate transporter 1 (SLC16A1) and glucagon (GCG) was induced by RS. In conclusion, our data show that RS modulates microbiota composition, SCFA concentrations, and host gene expression in pig intestine. Combined, our data provide an enhanced understanding of the interaction between diet, microbiota, and host
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