Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 435017
Title Virus-like particle nanoreactors: programmed en capsulation of the thermostable CelB glycosidase inside the P22 capsid
Author(s) Patterson, D.P.; Schwarz, B.; El-Boubbou, K.; Oost, J. van der; Prevelige, P.E.; Douglas, T.
Source Soft Matter 8 (2012)39. - ISSN 1744-683X - p. 10158 - 10166.
Department(s) Microbiological Laboratory
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) beta-glucosidase celb - archaeon pyrococcus-furiosus - protein cages - scaffolding protein - bacteriophage p22 - silica immobilization - enzyme immobilization - gold nanoparticles - functional domains - escherichia-coli
Abstract Self-assembling biological systems hold great potential for the synthetic construction of new active soft nanomaterials. Here we demonstrate the hierarchical bottom-up assembly of bacteriophage P22 virus-like particles (VLPs) that encapsulate the thermostable CelB glycosidase creating catalytically active nanoreactors. The in vivo assembly and encapsulation produces P22 VLPs with a high packaging density of the tetrameric CelB, but without loss of enzyme activity or the ability of the P22 VLP to undergo unique morphological transitions that modify the VLPs internal volume and shell porosity. The P22 VLPs encapsulating CelB are also shown to retain a high percentage of the enzyme activity upon being embedded and immobilized in a polymeric matrix
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