Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Record number 435351
Title Artemisinin production and precursor ratio in full grown Artemisia annua L. plants subjected to external stress
Author(s) Kjaer, A.; Verstappen, F.W.A.; Bouwmeester, H.J.; Ivarsen, E.; Frette, X.; Christensen, L.P.; Grevsen, K.; Jensen, M.
Source Planta 237 (2013). - ISSN 0032-0935 - p. 955 - 966.
Department(s) Laboratory of Plant Physiology
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) hairy root cultures - dihydroartemisinic acid - glandular trichomes - gene-expression - biosynthesis - accumulation - leaves - cells - identification - improvement
Abstract The concentration of the lifesaving antimalarial compound artemisinin (AN) in cultivated Artemisia annua (A. annua) plants is relatively low, and thus research in improving the content is important. In the present study, external stress was applied to adult plants of A. annua and the effect was examined on the concentrations of AN and its immediate precursors in leaves, and these concentrations were related to densities and sizes of the glandular trichomes (GT). Plants were stress treated weekly five times by sandblasting or spraying with salicylic acid, chitosan oligosaccharide, H2O2, and NaCl solutions. Contents of AN-related compounds (AN-c) were analysed in leaf samples from an upper and a lower position of the plants, and GT were quantified and measured. In lower leaves, several stress treatments had significant negative effects on concentrations of AN-c, whereas the ratios between compounds showed an increased conversion to AN. In the upper leaves, no changes were observed compared to controls. Linear relations were found between the concentrations of metabolites and the density of GT in both upper and lower leaves, and size of GT in lower leaves. Results suggested that older and younger leaves may respond differently to applied stress. A part of the plants were infected by powdery mildew, and this caused significantly different compositions of the AN-c, compared to uninfected plants. In conclusion, changes in concentrations of AN-c seemed largely to be related to changes in GT densities and sizes.
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