Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 435683
Title Structure of a post-translationally processed heterodimeric double-headed Kunitz-type serineprotease inhibitor from potato
Author(s) Meulenbroek, E.M.; Thomassen, E.A.J.; Pouvreau, L.A.M.; Abrahams, J.P.; Gruppen, H.; Pannu, N.S.
Source Acta Crystallographica Section D-Biological Crystallography 68 (2012)7. - ISSN 0907-4449 - p. 794 - 799.
Department(s) Food Chemistry Group
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) macromolecular crystallography - chymotrypsin inhibitor - molecular-replacement - proteinase-inhibitors - crystal-structure - reactive-site - cv elkana - refinement - seeds
Abstract Potato serine protease inhibitor (PSPI) constitutes about 22% of the total amount of proteins in potato tubers (cv. Elkana), making it the most abundant protease inhibitor in the plant. PSPI is a heterodimeric double-headed Kunitz-type serine protease inhibitor that can tightly and simultaneously bind two serine proteases by mimicking the substrate of the enzyme with its reactive-site loops. Here, the crystal structure of PSPI is reported, representing the first heterodimeric doubleheaded Kunitz-type serine protease inhibitor structure to be determined. PSPI has a-trefoil fold and, based on the structure, two reactive-site loops bearing residues Phe75 and Lys95 were identified.
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