Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 436308
Title Secretion of elastin-like polypeptides with different transition temperatures by Pichia pastoris
Author(s) Schipperus, R.; Eggink, G.; Wolf, F.A. de
Source Biotechnology Progress 28 (2012)1. - ISSN 8756-7938 - p. 242 - 247.
DOI https://doi.org/10.1002/btpr.717
Department(s) FBR Bioconversion
Bioprocess Engineering
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) protein - fusion - hydrophobicity - expression - hydrogels - purification - length
Abstract Like natural tropoelastin, polypeptides based on an elastin-like VPGXG repeat have a characteristic inverse temperature response, which leads to coacervate formation above a certain transition temperature and which could be useful for a variety of applications. The key advantage of elastin-like polypeptides (ELPs) over (tropo)elastin is a full control over this temperature response by adjustment of either the amino acid composition or the chain length, according to insights provided by extensive research. Future application of ELPs will require efficient ELP production systems, and in a previous article, we described the successful use of Pichia pastoris for secreted production of an ELP, with an overall yield of ˜ 200 mg L(-1). In this study, we investigated the influence of changed amino acid composition and chain length on the yield of secreted ELP. We have found that both parameters have a distinct impact on the overall yield, with higher yield for shorter and more hydrophilic ELPs. Because yield and transition temperature (Tt) thus appear to be positively correlated, we hypothesize that good solubility of ELP below the Tt promotes the secreted production and coacervate formation above Tt decreases it.
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