Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 437068
Title The biology of milk synthesis from a proteomics perspective
Author(s) Lu, J.
Source University. Promotor(en): Toon van Hooijdonk, co-promotor(en): Kasper Hettinga; Jacques Vervoort. - S.l. : s.n. - ISBN 9789461734600 - 177
Department(s) Product Design and Quality Management Group
Biochemistry
VLAG
Publication type Dissertation, internally prepared
Publication year 2013
Keyword(s) melksynthese - melksecretie - melksamenstelling - oppervlakte-eiwitten - eiwitexpressieanalyse - variatie - milk synthesis - milk secretion - milk composition - surface proteins - proteomics - variation
Categories Proteomics / Dairy Science
Abstract

Large variation in bovine milk composition of Dutch Holstein cows has been observed. The factors influencing the milk synthesis and secretion process in the mammary gland and the variations in this process lead to variation in milk composition. The understanding of milk synthesis was improved during the last decades, however, much is still unknown, especially with regard to lipid synthesis and secretion. In this research, a proteomics technique (FASP-Dimethyl labeling-NanoLC-Orbitrap-MS/MS) was used to identify and quantify low abundant proteins in milk. By using this proteomics technique combined with a metabolomics technique (1H-NMR), the variation in milk synthesis and secretion processes were shown as affected by several factors influencing milk composition: DGAT1 polymorphism, fat globule size and energy balance of the cow. Stomatin, cholesterol and cholesterol synthesis enzymes were shown to be important for milk synthesis and secretion in lactating cows in all the different studies. Cytoplasmic vesicle and cytoskeleton proteins were shown to be involved in milk fat globule secretion. These results led to new insights in milk synthesis and secretion.

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