Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 438128
Title Primary Separation: 2-D Electrophoresis
Author(s) Pedreschi Plasencia, R.P.
Source In: Proteomics in Foods: Principles and Applications / Toldrá, F., Nollet, L.M.L., New York, USA : Springer (Food Microbiology and Food Safety 2) - p. 51 - 59.
DOI https://doi.org/10.1007/978-1-4614-5626-1_3
Department(s) FBR Fresh Supply Chains
Publication type Chapter in scientific book
Publication year 2013
Abstract The advancements in two-dimensional electrophoresis (2DE) have consolidated it as a key tool for gel-based proteomics applications. Nowadays, 2DE is extensively applied and it is a useful technique for the simultaneous separation of hundreds to thousands of proteins, analysis, and differential quantification especially for the study of nonmodel organisms. This chapter focuses on all the steps involved in standard 2DE in protein expression. The pros and cons of the technique are discussed as well as potential variants for special applications. Special attention is given to data pre-processing and statistical analysis of 2DE generated data in order to draw sound conclusions from biological studies.
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