Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 438352
Title Carp neutrophilic granulocyes form extracellular traps via ROS-dependent and independent pathways
Author(s) Pijanowski, L.; Golbach, L.A.; Kolaczkowska, E.; Scheer, M.H.; Verburg-van Kemenade, B.M.L.; Chadzinska, M.K.
Source Fish and Shellfish Immunology 34 (2013)5. - ISSN 1050-4648 - p. 1244 - 1252.
Department(s) Cell Biology and Immunology
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) differential expression - net formation - teleost fish - myeloperoxidase - bacterial - immunity - genes - l. - matrix-metalloproteinase-9 - streptococcus
Abstract Neutrophil extracellular traps (NETs) have recently been described as an important innate defense mechanism that leads to immobilization and killing of invading pathogens. NETs have been identified in several species, but the mechanisms involved in NET formation and their role in infection have not been well determined yet. Here we show that upon in vitro stimulation with different immunostimulants of bacterial, fungal or viral origin, carp neutrophilic granulocytes rapidly release NET structures. We analyzed the composition of these structures and the kinetics of their formation by confocal microscopy, by quantifying the levels of extracellular DNA and the release of enzymes originating from neutrophilic granules: myeloperoxidase, neutrophil elastase and matrix metalloproteinase 9 (MMP-9). Profiles of NET release by carp neutrophils as well as their enzyme composition are stimulus- and time-dependent. This study moreover provides evidence for a stimulus-dependent selective requirement of reactive oxygen species in the process of NET formation. Collectively the results support an evolutionary conserved and strictly regulated mechanism of NET formation in teleost fish.
There are no comments yet. You can post the first one!
Post a comment
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.