Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 438754
Title eQTL mapping of candidate genes for flower colour as a model for genetical genomics in Azalea
Author(s) Keyser, E. de; Smulders, M.J.M.; Bockstaele, E. van; Riek, J. de
Source In: Proceedings of the 19th Eucarpia General Congress, 21-24 May 2012, Budapest, Hungary. - - p. 102 - 105.
Event 19th Eucarpia General Congress, Budapest, Hungary, 2012-05-21/2012-05-24
Department(s) WUR Plant Breeding
EPS
Publication type Contribution in proceedings
Publication year 2012
Abstract Flower colour in azalea is inherited as a semi-qualitative trait and is mainly determined by differences in anthocyanins and flavonols. A two-gene model is used to explain the phenotypic variation between white, brick red and carmine red colour: W in case the flower petals contain anthocyanins and Q if flavonols are present as co-pigments. However, the regulatory network behind this pathway is still unclear. To identify key driver genes of the molecular pathway involved, a genetical genomics approach was followed. Genetics was combined with gene expression profiling, resulting in eQTL mapping. So far, mainly micro-array data have been used for this, limiting the technique to large scale projects. Nevertheless, qPCR can be a good and cost?efficient alternative, as we will demonstrate for flower colour in azalea. A genetic map of 13 linkage groups was constructed using a crossing population segregating for flower colour (250 plants). Besides anonymous AFLP and SSR markers also a set of functional markers
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