Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 439093
Title Forensic Identifiation of Indian Snakeroot (Rauvolfia serpentina Benth. ex Kurz) Using DNA Barcoding.
Author(s) Eurlings, M.C.M.; Lens, F.; Pakusza, C.; Peelen, T.; Wieringa, J.J.; Gravendeel, B.
Source Journal of Forensic sciences 58 (2013)3. - ISSN 0022-1198 - p. 822 - 830.
DOI https://doi.org/10.1111/1556-4029.12072
Department(s) Biosystematics
EPS
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) pcr-rflp method - molecular-identification - apocynaceae s.l. - ancient dna - plants - phylogeny - intron
Abstract Indian snakeroot (Rauvolfia serpentina) is a valuable forest product, root extracts of which are used as an antihypertensive drug. Increasing demand led to overharvesting in the wild. Control of international trade is hampered by the inability to identify root samples to the species level. We therefore evaluated the potential of molecular identification by searching for species-specific DNA polymorphisms. We found two species-specific indels in the rps16 intron region for R. serpentina. Our DNA barcoding method was tested for its specificity, reproducibility, sensitivity and stability. We included samples of various tissues and ages, which had been treated differently for preservation. DNA extractions were tested in a range of amplification settings and dilutions. Species-specific rps16 intron sequences were obtained from 79 herbarium accessions and one confiscated root, encompassing 39 different species. Our results demonstrate that molecular analysis provides new perspectives for forensic identification of Indian snakeroot.
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