Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 439147
Title Isolation of polymorphic microsatellite markers and tests of cross-amplification in four widespread European calcicole ferns
Author(s) Groot, G.A. de; Korpelainen, H.; Wubs, E.R.J.; Erkens, R.H.J.
Source American Journal of Botany 98 (2011)11. - ISSN 0002-9122 - p. e319 - e322.
Department(s) Alterra - Animal ecology
Laboratory of Nematology
Publication type Refereed Article in a scientific journal
Publication year 2011
Abstract Premise of the study: Studies on the biogeography and population genetics of the widespread European rock ferns Asplenium scolopendrium , A. trichomanes subsp. quadrivalens , Polystichum setiferum , and P. aculeatum would potentially yield interesting new insights into the colonization capacities of ferns. Markers with suffi cient resolution for detailed genetic studies are, however, not yet available. Methods and Results: Using genome screening with intersimple sequence repeat (ISSR) primers, a total of 16 different microsatellite markers were isolated and characterized for the four species. Some of these markers could be exchanged within each congeneric pair. Conclusions: The developed primer sets will be very useful for analyses of the biogeography and population genetics of some widespread calcicole ferns. The observed cross-amplifi cation rates suggest a high potential for application on additional species from the same genera
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