Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 439571
Title The mechanism of introner-like element multiplication in fungi
Author(s) Burgt, I.A. van der; Severing, E.I.; Ochoa Tufiño, V.; Wit, P.J.G.M. de; Collemare, J.A.R.
Source In: Book of Abstracts 27th Fungal Genetics Conference, Asilomar, Pacific Grove, California, USA, 12-17 March 2013. - - p. 205 - 205.
Event 27th Fungal Genetics Conference, 2013-03-12/2013-03-17
Department(s) Laboratory of Phytopathology
Bioinformatics
EPS-2
Publication type Abstract in scientific journal or proceedings
Publication year 2013
Abstract The recent discovery of introner-like elements (ILEs) in six fungal species shed new light on the origin of regular spliceosomal introns (RSIs). ILEs are novel spliceosomal introns that are found in hundreds of near-identical copies in unrelated genes. They account for the vast majority of intron gains in these species and are not associated with intron losses. Remarkably, ILEs are longer than RSIs and harbor predicted stable secondary structures. However, they are prone to quickly degenerate in sequence and length to become undistinguishable from RSIs, suggesting that ILEs are predecessors of most RSIs. Further analyses are being performed in order to understand the multiplication mechanism of ILEs, which is hypothesized to resemble the retro-homing mechanism of self-splicing group II introns. The dynamics of ILE’s secondary structures could be predicted and two conserved motifs were identified in almost all fungal ILEs, which might play an important role in direct insertion into DNA. We also have developed a genetic screen in yeast in order to capture and characterize ILE insertion events. These ongoing studies should provide hints about the mechanism of ILE multiplication, i.e. how new spliceosomal introns are gained in fungi.
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