Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 440084
Title Microsphere-based binding assays for organic pollutants
Author(s) Meimaridou, A.
Source University. Promotor(en): Michel Nielen, co-promotor(en): Willem Haasnoot. - S.l. : s.n. - ISBN 9789461735768 - 139
Department(s) RIKILT - BU Toxicology Bioassays & Novel Foods
RIKILT Directie
VLAG
Publication type Dissertation, internally prepared
Publication year 2013
Keyword(s) persistente organische verontreinigende stoffen - toxische stoffen - vis - schildklierhormonen - doorstroomcytometrie - screenen - assays - persistent organic pollutants - toxic substances - fish - thyroid hormones - flow cytometry - screening
Categories Environmental Toxicology, Ecotoxicology
Abstract

In this thesis the “proof of principle” of a preliminary “prototype kit” for the screening of dioxin-like PCBs, PBDEs and PAHs in fish combined with two different simplified generic cleanup procedures, according to the fish fat content, is presented. Toxicants such as the PCBs, PBDEs and PAHs and POPs mixtures (such as the technical mixtures of PCBs (Aroclors)) can be detected at relative low levels (with IC50 values 55±15, 2±0.4 and 4±0.5 ppb for the PCB77, PBDE47 and BaP, respectively) in fish samples in a multiplex, simple and inexpensive manner compared to existing techniques. The 3-plex immunoassay can be performed in two different detection platforms: the traditional flow cytometer- and the new imaging-based bead analyzers. The latter system offers a cheaper analysis and it is an easier transportable platform than the flow cytometer.

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