Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 440835
Title Diversity of human small intestinal Streptococcus and Veillonella populations
Author(s) Bogert, B. van den; Erkus, O.; Boekhorst, J. te; Goffau, M.; Smid, E.J.; Zoetendal, E.G.; Kleerebezem, M.
Source FEMS Microbiology Ecology 85 (2013)2. - ISSN 0168-6496 - p. 376 - 388.
DOI http://dx.doi.org/10.1111/1574-6941.12127
Department(s) Microbiological Laboratory
Food Microbiology Laboratory
Host Microbe Interactomics
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) human gastrointestinal-tract - multiple sequence alignment - desorption ionization-time - flight mass-spectrometry - high-throughput - gut microbiome - molecular analysis - genetic diversity - immune-responses - oral-cavity
Abstract Molecular and cultivation approaches were employed to study the phylogenetic richness and temporal dynamics of Streptococcus and Veillonella populations in the small intestine. Microbial profiling of human small intestinal samples collected from four ileostomy subjects at four time points displayed abundant populations of Streptococcus spp. most affiliated with S. salivarius, S. thermophilus, and S. parasanguinis, as well as Veillonella spp. affiliated with V. atypica, V. parvula, V. dispar, and V. rogosae. Relative abundances varied per subject and time of sampling. Streptococcus and Veillonella isolates were cultured using selective media from ileostoma effluent samples collected at two time points from a single subject. The richness of the Streptococcus and Veillonella isolates was assessed at species and strain level by 16S rRNA gene sequencing and genetic fingerprinting, respectively. A total of 160 Streptococcus and 37 Veillonella isolates were obtained. Genetic fingerprinting differentiated seven Streptococcus lineages from ileostoma effluent, illustrating the strain richness within this ecosystem. The Veillonella isolates were represented by a single phylotype. Our study demonstrated that the small intestinal Streptococcus populations displayed considerable changes over time at the genetic lineage level because only representative strains of a single Streptococcus lineage could be cultivated from ileostoma effluent at both time points
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