Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 440869
Title Genome Analysis and Physiological Comparison of Alicycliphilus denitrificans Strains BC and K601(T.)
Author(s) Oosterkamp, M.J.; Veuskens, T.; Talarico Saia, F.; Weelink, S.A.B.; Goodwin, L.A.; Daligault, H.E.; Bruce, D.C.; Detter, J.C.; Tapia, R.; Han, C.S.; Land, M.; Hauser, L.; Langenhoff, A.A.M.; Gerritse, J.; Berkel, W.J.H. van; Pieper, D.H.; Junca, H.; Smidt, H.; Schraa, G.; Davids, M.; Schaap, P.J.; Plugge, C.M.; Stams, A.J.M.
Source PLoS One 8 (2013)6. - ISSN 1932-6203
DOI http://dx.doi.org/10.1371/journal.pone.0066971
Department(s) Microbiological Laboratory
Sub-department of Environmental Technology
Biochemistry
Systems and Synthetic Biology
VLAG
WIMEK
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) periplasmic nitrate reductase - anaerobic benzene degradation - cytochrome-c-oxidase - aromatic-compounds - perchlorate reduction - chlorite dismutase - reducing bacteria - rna genes - 1st step - metabolism
Abstract The genomes of the Betaproteobacteria Alicycliphilus denitrificans strains BC and K601(T) have been sequenced to get insight into the physiology of the two strains. Strain BC degrades benzene with chlorate as electron acceptor. The cyclohexanol-degrading denitrifying strain K601(T) is not able to use chlorate as electron acceptor, while strain BC cannot degrade cyclohexanol. The 16S rRNA sequences of strains BC and K601(T) are identical and the fatty acid methyl ester patterns of the strains are similar. Basic Local Alignment Search Tool (BLAST) analysis of predicted open reading frames of both strains showed most hits with Acidovorax sp. JS42, a bacterium that degrades nitro-aromatics. The genomes include strain-specific plasmids (pAlide201 in strain K601(T) and pAlide01 and pAlide02 in strain BC). Key genes of chlorate reduction in strain BC were located on a 120 kb megaplasmid (pAlide01), which was absent in strain K601(T). Genes involved in cyclohexanol degradation were only found in strain K601(T). Benzene and toluene are degraded via oxygenase-mediated pathways in both strains. Genes involved in the meta-cleavage pathway of catechol are present in the genomes of both strains. Strain BC also contains all genes of the ortho-cleavage pathway. The large number of mono- and dioxygenase genes in the genomes suggests that the two strains have a broader substrate range than known thus far
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