Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 441003
Title Variation in microbial responses and Rhizoctonia solani AG2-2IIIB growth in soil under different organic amendment regimes
Author(s) Overbeek, L.S. van; Senechkin, I.; Bruggen, A.H.C. van
Source Canadian Journal of Plant Pathology = Revue Canadienne de Phytopathologie 34 (2012)2. - ISSN 0706-0661 - p. 268 - 276.
DOI https://doi.org/10.1080/07060661.2012.679622
Department(s) PRI BIOINT Ecological Interactions
Centre for Genetic Resources, The Netherlands
Biological Farming Systems
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) gradient gel-electrophoresis - ammonia-oxidizing bacteria - 16s ribosomal-rna - ralstonia-solanacearum biovar-2 - fluorescent pseudomonads - nitrogen fertilizers - disease suppression - soilborne diseases - take-all - communities
Abstract Organic amendments influence chemical and microbial compositions in soils and also susceptibility to plant diseases. The purpose of this study was to establish different parameters that interfere with pathogen growth in soil. Four different organic-amendment regimes, i.e. slurry, compost, slurry-dung and compost-slurry-dung, were applied to two fields proximate to each other on the same farm and covered with the same grass-clover ley in the last two years preceding sampling. Before that period, there were differences in cropping and management practices between both fields. Chemical analyses of the soils revealed no differences between organic amendments, whereas significant differences were present between fields in all C, N and pH values. Growth of R. solani AG2-2IIIB in soil, measured by damping-off in sugar beet plants, was influenced by the interaction of organic amendment with field type. Diversity and evenness values of the microbial communities, studied by PCR-DGGEs specific for bacteria, fungi, Pseudomonas and ammonia-oxidizing beta-proteobacteria, revealed different patterns, i.e. no differences between organic amendments, but clear differences between fields. Multivariate analyses done on individual species of the four groups, as represented by band location and size in PCR-DGGE fingerprints, and by inclusion of chemical and R. solani AG2-2IIIB growth parameters as `environmental' variables, revealed strong and occasionally significant effects of organic matter content, water-dissolvable organic carbon and pH on microbial communities. It was therefore concluded that different organic amendments had different effects on pathogen growth in soils of both fields and that organic matter content and pH influenced soil microbial compositions most.
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