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Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Record number 442681
Title Characterisation of cell wall polysaccharides from rapeseed (Brassica napus) meal
Author(s) Pustjens, A.M.; Schols, H.A.; Kabel, M.A.; Gruppen, H.
Source Carbohydrate Polymers 98 (2013)2. - ISSN 0144-8617 - p. 1650 - 1656.
Department(s) Food Chemistry Group
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) campestris polysaccharides - nonstarch polysaccharides - structural-characterization - aspergillus-aculeatus - liquid-chromatography - rhamnogalacturonan-i - pectic substances - purification - xyloglucan - oligosaccharides
Abstract To enable structural characteristics of individual cell wall polysaccharides from rapeseed (Brassica napus) meal (RSM) to be studied, polysaccharide fractions were sequentially extracted. Fractions were analysed for their carbohydrate (linkage) composition and polysaccharide structures were also studied by enzymatic fingerprinting. The RSM fractions analysed contained pectic polysaccharides: homogalacturonan in which 60% of the galacturonic acid residues are methyl-esterified, arabinan branched at the O-2 position and arabinogalactan mainly type II. This differs from characteristics previously reported for Brassica campestris meal, another rapeseed cultivar. Also, in the alkali extracts hemicelluloses were analysed as xyloglucan both of the XXGG- and XXXG-type decorated with galactosyl, fucosyl and arabinosyl residues, and as xylan with O-methyl-uronic acid attached. The final residue after extraction still contained xyloglucan and remaining (pectic) polysaccharides next to cellulose, showing that the cell wall matrix of RSM is very strongly interconnected
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