Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 442682
Title Light harvesting and Blue-Green light induced non-photochemical quenching in two different C-phycocyanin mutants of synechocytis PCC 6803
Author(s) Tian, L.; Stokkum, I.H.M. van; Koehorst, R.B.M.; Amerongen, H. van
Source The Journal of Physical Chemistry Part B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical 117 (2013)38. - ISSN 1520-6106 - p. 11000 - 11006.
Department(s) Biophysics
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) orange carotenoid protein - excitation-energy transfer - photosystem-ii - sp pcc-6803 - phycobilisome fluorescence - photoprotective mechanism - picosecond kinetics - wild-type - cyanobacterium - photosynthesis
Abstract Cyanobacteria are oxygen-evolving photosynthetic organisms that harvest sunlight and convert excitation energy into chemical energy. Most of the light is absorbed by large light harvesting complexes called phycobilisomes (PBs). In high-light conditions, cyanobacteria switch on a photoprotective mechanism called non-photochemical quenching (NPQ): During this process, absorption of blue-green light transforms the inactive orange form of the orange carotenoid protein OCP (OCPo) into the red active form OCPr that subsequently binds to the PB, resulting in a substantial loss of excitation energy and corresponding decrease of the fluorescence. In wild-type cells, the quenching site is a bilin chomophore that fluoresces at 660 nm and which is called APCQ660. In the present work, we studied NPQ in two different types of mutant cells (CB and CK) that possess significantly truncated PBs, using spectrally resolved picosecond fluorescence spectroscopy. The results are in very good agreement with earlier in vitro experiments on quenched and unquenched PBs, although the fraction of quenched PBs is far lower in vivo. It is also lower than the fraction of PBs that is quenched in wild-type cells, but the site, rate, and location of quenching appear to be very similar.
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