Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 443119
Title Effect of nitrogen sources on in vitro fermentation profiles and microbial yield using equine caecal contents
Author(s) Santos, A.S.; Ferreira, L.M.M.; Martin-Rosset, W.; Cone, J.W.; Bessa, R.J.B.; Rodrigues, M.A.M.
Source Animal Feed Science and Technology 182 (2013)1-4. - ISSN 0377-8401 - p. 93 - 99.
Department(s) Animal Nutrition
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) gas-production profiles - protein fermentation - streptococcus-bovis - rumen - growth - bacteria - acid - efficiency - kinetics - balance
Abstract The effect of different nitrogen and carbohydrate sources on in vitro fermentation profile and microbial yield of equine caecal contents was assessed. For this purpose, caecal contents were collected from 3 geldings, fed at maintenance level twice a day, and diluted with a buffer mineral solution (1:10, v/v). Caecal inoculum was then used in incubationsusing the gas production technique in one of the following treatments: mixture of rapidlyfermentable carbohydrates (glucose, xylose and starch); 25 mg of N in the form of casein; 25 mg of N in the form of urea; mixture of rapidly fermentable carbohydrates plus 25 mg of N in the form of casein; mixture of rapidly fermentable carbohydrates plus 25 mg of N in the form of urea; no substrate. Total volatile fatty acids (VFA), ammonia nitrogen and gas production were measured after a 24-h incubation. Microbial biomass was estimated using adenine and guanine bases as internal markers, and microbial growth efficiency (YATP) and gas efficiency (Egas) were estimated. Results showed a higher fermentative activity when nitrogen together with soluble carbohydrates was provided in contrast to treatments that were energy or nitrogen limited. When nitrogen and carbohydrates are provided, besides an efficient growth of the microbial population, there is a marked increase in total VFA and gas production. Although the caecal microbial population will respond to casein addition with an increase in VFA and gas production, microbial growth efficiency will be lower compared to the addition of urea as nitrogen source, indicating that the microbial population would mainly use non protein nitrogen for growth.
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