Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 443491
Title Multiplex detection and identification of bacterial pathogens causing potato blackleg and soft rot in Europe, using padlock probes
Author(s) Slawiak, M.; Doorn, R. van; Szemes, M.; Speksnijder, A.G.C.L.; Waleron, M.; Wolf, J.M. van der; Lojkowska, E.; Schoen, C.D.
Source Annals of Applied Biology 163 (2013)3. - ISSN 0003-4746 - p. 378 - 393.
Department(s) PRI BIOINT Ecological Interactions
Biointeracties and Plant Health
PRI BIOINT Moleculair Phytopathology
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) fragment-length-polymorphism - carotovora ssp-atroseptica - erwinia-carotovora - pectobacterium-carotovorum - sp-nov. - oligonucleotide-microarray - dickeya-chrysanthemi - genetic diversity - pectin lyase - new-zealand
Abstract The objective of this study was to develop a multiplex detection and identification protocol for bacterial soft rot coliforms, namely Pectobacterium wasabiae (Pw), Pectobacterium atrosepticum (Pba) and Dickeya spp., responsible for potato blackleg and tuber soft rot. The procedures were derived from the phylogenetic relationships of these and other Enterobacteriaceae based on recA sequences. The group of Pw strains was highly homogeneous and could be distinguished from the other species. A ligation-based method for detection of Pw was developed. Five padlock probes (PLPs) were designed, targeting recA sequences to identify the Pw, Pba or Dickeya spp., whereas a sixth probe recognised recA sequences of all soft rot coliforms including Pectobacterium carotovorum subsp. carotovorum (Pcc). Two PLP-based applications were developed: one using real-time PCR and one using universal microarrays. Assay sensitivity and specificity were demonstrated using 71 strains of Pw, Pcc, Pba and Dickeya spp. Both multiplex methods can be potentially used for seed testing and in ecological studies, but further validation is required
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