Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 443617
Title Expression of the dyslexia candidate gene kiaa0319-like in insect cells
Author(s) Holster, S.; Oers, M.M. van; Roode, E.C.; Tsang, O.W.H.; Yeung, V.S.Y.; Vlak, J.M.; Waye, M.M.Y.
Source Journal of Biochemistry and Molecular Biology in The Post Genomic Era 2 (2013)1. - ISSN 2156-5732 - p. 45 - 52.
Department(s) PE&RC
Laboratory of Virology
EPS
Publication type Refereed Article in a scientific journal
Publication year 2013
Abstract The human kiaa0319-like gene is one of the candidate genes for developmental dyslexia, but the exact function of the encoded KIAA0319L (KL) protein is not known. To allow functional analysis a purified, biologically active KL protein is required. The kiaa0319-like gene was expressed in insect cells using the baculovirus expression system. To optimize the expression of the kiaa0319-like gene and to be able to purify the KL protein, several approaches were used. Two different recombinant baculoviruses were made, one with the full length coding sequence of KL and one that lacked the transmembrane domain to facilitate purification.. Versions in which the kiaa0319L sequences were cloned downstream of the honeybee melittin signal sequence were also made. All four constructs contained a C-terminal influenza hemagglutinin (HA)-tag. Sf9 insect cells infected with these recombinant baculoviruses produced the KL protein, as demonstrated by Western blot analysis using either the HA-antibody or KL-specific polyclonal serum.
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