Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 443864
Title Bead-based DNA assay for the detection od Streptococcus suis in tonsillar specimens of pigs
Author(s) Wal, F.J. van der; Wisselink, H.J.; Bergervoet, J.; Weerdt, M. de; Achterberg, R.P.
Source In: Med-Vet-Net Association International Scientific Conference DTU, Lyngby, Denmark, 24-25 June 2013: Abstract Book. Lyngby, 2013. - - p. 27 - 27.
Event Med-Vet-Net Association International Scientific Conference DTU, Lyngby, 2013-06-24/2013-06-25
Department(s) CVI Infection Biology
PRI BIOINT Moleculair Phytopathology
Publication type Abstract in scientific journal or proceedings
Publication year 2013
Abstract Streptococcus suis is an important swine pathogen in nearly all countries with an extensive pig industry. In pigs it is associated with meningitis, arthritis, endocarditis, septicemia, pneumonia and sudden death. In certain parts of Asia S. suis is a common cause of bacterial meningitis in humans. Attempts to control S. suis in pig farming are hampered by the lack of sensitive diagnostic tools and effective vaccines. To provide a basis for adequate control measures, a bead-based suspension array was developed that targets six genes using multiplex PCR followed by target specific primer extension (TSPE) and subsequent hybridisation to beads. Assays were designed using AlleleID and Primer- Plex on consensus sequences of four serotype specific capsular polysaccharide (cps) genes (cps1I, cps2J, cps7H, cps9H), the extracellular virulence factor EF (epf) and a general S. suis marker (glutamate dehydrogenase; gdh). The proof-of-principle of the assay was demonstrated with chromosomal DNA of reference strains: each PCR was capable of producing the desired amplicon, also when performed in a sixplex reaction. Upon hybridisation of the TSPE products, all multiplex PCR products produced signals on the appropriate beads. This assay will be evaluated with field samples, using DNA isolated from tonsillar specimens of pigs. Ultimately, this expandable assay may be applicable for studying epidemiology and transmission, and could contribute to efforts aimed at control and eradication of S. suis in pig farming, thereby contributing to human health.
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