Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 444867
Title Linkage mapping in the oilseed crop Jatropha curcas L. reveals a locus controlling the biosynthesis of phorbol esters which cause seed toxicity
Author(s) King, A.J.; Montes, L.R.; Clarke, J.G.; Affleck, J.; Li, Y.; Witsenboer, H.; Vossen, E. van der; Linde, P. van der; Tripathi, Y.; Tavares, E.; Shukla, P.; Rajasekaran, T.; Loo, E.N. van; Graham, I.A.
Source Plant Biotechnology Journal 11 (2013)8. - ISSN 1467-7644 - p. 986 - 996.
DOI https://doi.org/10.1111/pbi.12092
Department(s) Laboratory of Plant Breeding
PPO Paddestoelen
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) transcriptome analysis - genetic diversity - biofuel plant - genome - oil - markers - qtl - construction - sequence - maps
Abstract Current efforts to grow the tropical oilseed crop Jatropha curcas L. economically are hampered by the lack of cultivars and the presence of toxic phorbol esters (PE) within the seeds of most provenances. These PE restrict the conversion of seed cake into animal feed, although naturally occurring ‘nontoxic’ provenances exist which produce seed lacking PE. As an important step towards the development of genetically improved varieties of J. curcas, we constructed a linkage map from four F2 mapping populations. The consensus linkage map contains 502 codominant markers, distributed over 11 linkage groups, with a mean marker density of 1.8 cM per unique locus. Analysis of the inheritance of PE biosynthesis indicated that this is a maternally controlled dominant monogenic trait. This maternal control is due to biosynthesis of the PE occurring only within maternal tissues. The trait segregated 3 : 1 within seeds collected from F2 plants, and QTL analysis revealed that a locus on linkage group 8 was responsible for phorbol ester biosynthesis. By taking advantage of the draft genome assemblies of J. curcas and Ricinus communis (castor), a comparative mapping approach was used to develop additional markers to fine map this mutation within 2.3 cM. The linkage map provides a framework for the dissection of agronomic traits in J. curcas, and the development of improved varieties by marker-assisted breeding. The identification of the locus responsible for PE biosynthesis means that it is now possible to rapidly breed new nontoxic varieties.
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