Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 445482
Title The Large Scale Identification and Quantification of Conjugates of Intact and Gut Microbial Bioconversion Products of Polyphenols.
Author(s) Hooft, J.J.J. van der; Vos, C.H. de; Bino, R.J.; Mihaleva, V.V.; Ridder, L.O.; Roo, N. de; Jacobs, D.M.; Duynhoven, J.P.M. van; Vervoort, J.
Source In: Magnetic Resonance in Food Science / Duynhoven, J., Belton, P.S., Webb, G.A., As, H., CBSG/NMC - ISBN 9781849736343 - p. 177 - 182.
Department(s) PRI BIOS Applied Metabolic Systems
Laboratory of Plant Physiology
AFSG Staff Departments (FBR)
Biochemistry
Biophysics
VLAG
Publication type Peer reviewed book chapter
Publication year 2013
Abstract A human diet containing a significant amount of flavonoids, such as present in tea, red wine, apple, and cocoa has been associated with reduced disease risks. After consumption, a part of these flavonoids can be directly absorbed by the small intestine, but the greatest part passages towards the large intestine where microbes break the flavonoids down into phenolic metabolites. After absorption into the blood, both intact and metabolized flavonoids are subsequently methylated, sulphated, and glucuronidated or a combination thereof. The exact chemical structural elucidation and quantification of these conjugates present in the human body are key to identify potential bioactive components. However, this is still a tedious task due to their relative low abundance in a complex background of other high-abundant metabolites and the many possible isomeric forms. Therefore, we aimed to systematically identify these conjugates by using a combination of pre-concentration and separation by solid phase extraction (SPE) followed by LC-FTMSn and 1D 1H NMR. The combination of LC-FTMSn and HPLC-TOF-MS-SPE-NMR resulted in the efficient identification and quantification of low abundant polyphenol metabolites down to micromolar concentrations and thus opens up new perspectives for in depth studying of the bioavailability and the possible mode of action of flavonoids like flavan-3-ols and their gut-microbial break-down products circulating in the human body.
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