Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 447374
Title Fluorescent In Situ Hybridization (FISH) on Pachytene Chromosomes as a Tool for Genome Characterization. In: Legume Genomics
Author(s) Geurts, R.; Jong, J.H.S.G.M. de
Source In: Legume Genomics: Methods and Protocols, Methods in Molecular Biology. Volume 1069 / Rose, R.J., Springer Science + Business Media - ISBN 9781627036122 - p. 15 - 24.
Department(s) Laboratory of Molecular Biology
Laboratory of Genetics
Publication type Peer reviewed book chapter
Publication year 2013
Abstract A growing number of international genome consortia have initiated large-scale sequencing projects for most of the major crop species. This huge amount of information not only boosted genetic and physical mapping research, but it also enabled novel applications on the level of chromosome biology including molecular cytogenetics that supports plant genetics, genomics, and breeding programs. The simultaneous detection of a large number of BAC-based probes by multicolor fluorescent in situ hybridization (FISH) can provide a rapid overview of super-contig and gap distribution on euchromatin chromosome areas and will display directly and precisely the positions of chromosome rearrangements. Furthermore, hybridizations of BACs on the chromosomes of related species can confirm genomic colinearity, or the occurrence of inversions and translocations events. This cross-species FISH together with meiotic pairing studies is a powerful source of information that elucidates the nature of genome rearrangements, and the consequences of such rearrangements for introgressive hybridizations. In this chapter we describe a general-purpose protocol for FISH on pachytene chromosomes
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