Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 447585
Title Recovery of microbial diversity and activity during bioremediation following chemical oxidation of diesel contaminated soils
Author(s) Sutton, N.B.; Langenhoff, A.A.M.; Hidalgo Lasso, D.; Zaan, B.M. van der; Gaans, P. van; Maphosa, F.; Smidt, H.; Grotenhuis, J.T.C.; Rijnaarts, H.H.M.
Source Applied Microbiology and Biotechnology 98 (2014)6. - ISSN 0175-7598 - p. 2751 - 2764.
DOI https://doi.org/10.1007/s00253-013-5256-4
Department(s) Sub-department of Environmental Technology
Microbiological Laboratory
WIMEK
Publication type Refereed Article in a scientific journal
Publication year 2014
Keyword(s) polycyclic aromatic-hydrocarbons - gradient gel-electrophoresis - in-situ ozonation - real-time pcr - crude-oil - fentons reagent - biological remediation - community composition - organic-matter - ribosomal-rna
Abstract To improve the coupling of in situ chemical oxidation and in situ bioremediation, a systematic analysis was performed of the effect of chemical oxidation with Fenton's reagent, modified Fenton's reagent, permanganate, or persulfate, on microbial diversity and activity during 8 weeks of incubation in two diesel-contaminated soils (peat and fill). Chemical oxidant and soil type affected the microbial community diversity and biodegradation activity; however, this was only observed following treatment with Fenton's reagent and modified Fenton's reagent, and in the biotic control without oxidation. Differences in the highest overall removal efficiencies of 69 % for peat (biotic control) and 59 % for fill (Fenton's reagent) were partially explained by changes in contaminant soil properties upon oxidation. Molecular analysis of 16S rRNA and alkane monooxygenase (alkB) gene abundances indicated that oxidation with Fenton's reagent and modified Fenton's reagent negatively affected microbial abundance. However, regeneration occurred, and final relative alkB abundances were 1–2 orders of magnitude higher in chemically treated microcosms than in the biotic control. 16S rRNA gene fragment fingerprinting with DGGE and prominent band sequencing illuminated microbial community composition and diversity differences between treatments and identified a variety of phylotypes within Alpha-, Beta-, and Gammaproteobacteria. Understanding microbial community dynamics during coupled chemical oxidation and bioremediation is integral to improved biphasic field application.
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